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  • Name: Alina
  • Tel: +86 021 5990 3780/+86 150 5727 9866
  • Fax: +86 021 5990 3780
  • E-mail: sales@ylbiont.com
  • Add: No. 5588, Caoan Road, Jiading District, Shanghai

Product Name: ELISA Kit for mouse Bone Morphogenetic Protein 4 (BMP4)

Art No.: YLC1321MO

Application: ELISA

Size: 96T/48T

Reaction: Mus musculus mouse

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Method:sandwich

Detection range:0.156-10ng/mL

Sensitivity:0.057ng/mL

Assay length:1-4.5Hours

Detection Wavelengt:450nm

Research Area:Immunology

Price:inquiry

Quality guarantee period:for 12 months

Introduction


ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells2
Standard2Standard Diluent1×20mL
Detection Reagent A1×70μLAssay Diluent A1×12mL
Detection Reagent B1×120μLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

STORAGE OF THE KITS

1. For unopened kit: All the reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer (30 × concentrate) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at -20℃.

2. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.


INTENDED USE

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.


PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%  

Inter-Assay: CV<12%


ASSAY PROCEDURE SUMMARY

1. Prepare all reagents, samples and standards; 

2. Add 100μL standard or sample to each well. Incubate 2 hours at 37℃; 

3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37℃;

4. Aspirate and wash 3 times; 

5. Add 100μL prepared Detection Reagent B. Incubate 1hour at 37℃; 

6. Aspirate and wash 5 times; 

7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37℃; 

8. Add 50μL Stop Solution. Read at 450nm immediately. 


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  • Name: Alina
  • Tel: +86 021 5990 3780/+86 150 5727 9866
  • Fax: +86 021 5990 3780
  • E-mail: sales@ylbiont.com
  • Add: No. 5588, Caoan Road, Jiading District, Shanghai
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