Product Name: Rat Gasdermin D(GSDMD)ELISA Kit

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INTENDED USE

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma,tissue homogenates,cell lysates,cell culture supernates and other biological fluids.

STORAGE

For unopened kit: All the reagents should be kept according to the labels on vials, Washing concentrate (30X) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at -20℃

PRECISION

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<8%  

Inter-Assay: CV<10%

ASSAY PROCEDURE SUMMARY 

1. Prepare all reagents, samples and standards; 

2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.

3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at 37 ℃; for color development.

4. Add stop solution

5. Read the OD value within 10minutes. 

6. Calculate.

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Quantity

Reagents

Quantity

Coated ELISA plate 

12-Well * 8 Tubes 

Washing concentrate (30X) 

20ml 

Standard dilution 

3ml 

Instruction 

1 

Chromogen solution A  

6ml 

Seal plate membrane 

2 

Chromogen solution B 

6ml 

Hermetic bag 

1 

Streptavidin-HRP 

6ml 

Stop solution 

6ml 

Standard solution 

0.5ml 

Antibodies labeled with biotin 

1ml