Art No.: YLM0460HU
Application: CLIA
Size: 96T
Reaction: Human
Inquire NowMethod:sandwich
Detection range:62.5~4000pg/mL
Sensitivity:37.5pg/mL
Research Area:Immunology
Price:inquiry
Quality guarantee period:for 12 months
Introduction
Reagents | Quantity | save |
Micro CLIA Plate | 8×12 wells | 4℃/-20℃ |
Reference Standard | 2pcs | 4℃/-20℃ |
Reference Standard & Sample Diluent | 1bottle 20mL | 4℃ |
Concentrated Biotinylated Detection Ab | 1pcs 120μL | 4℃/-20℃ |
Biotinytated Detection Ab Diluent | 1bottle 10mL | 4℃ |
Concentrated HRP Conjugate | 1pcs 120μL | 4℃(lucifuge) |
HRP Conjugate Diluent | 1bottle 10mL | 4℃ |
Concentrated Wash Buffer (25×) | 1bottle 30mL | 4℃ |
Substrate Reagent A | 1bottle 5mL | 4℃(lucifuge) |
Substrate Reagent B | 1bottle 5mL | 4℃(lucifuge) |
Plate Sealer | 5pcs |
|
Manual | 1part |
|
Certificate of Analysis | 1part |
it's OK to keep the in 4℃,if the kit is scheduled to be used up in one week,
please keep the reagent in -20℃ for long-term storage or repeated use
specifieity
No,significant cross-reactivity or interference was observde
Repeatability
Coefficient of variation were<15%
SUMMARY
1.Add 100ul standard or sample to each well.Incubate 90mintues at 37℃
2.Remove the liquid Add 100ul Biotinylated Detection Ab.Incubate 1 hour at 37℃
3.Aspirate and wash 3 times
4.Add 100ul HRP Conjugate.Incubate 30 minutes at 37℃
5.Aspirate and wash 5 times
6.Add 100ul Substrate Mixture Solution.Invubate 5 minutes at 37℃
7.Read immediately after the subastrate reaction time
8.Calculation of results