Art No.: YLD8378
Application: WB, IHC, IP
Size: 50ul/100ul
Reaction: H, M, R
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| Background | Mouse monoclonal antibody to Alpha-actin-1 |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-actin-1. The exact sequence is proprietary. |
| WB description | Western blot analysis of Alpha-actin-1 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. |
| IHC description | Immunohistochemical analysis of Alpha-actin-1 staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | Immunoprecipitation of Alpha-actin-1 from 0.5mg mouse brain whole cell extract lysate, using anti-Alpha-actin-1 Antibody. |
| FC description | |
| ChIP description | |
| Purification_Method | |
| Clonality | Monoclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/5000 - 1/10000), IH (1/200 - 1/500), IP (1/100 - 1/200) |
| Gene ID(Human) | 58 |
| Gene ID (Mouse) | 11459 |
| Gene ID(Rat) | 29437 |
| SwissProt (Human) | P68133 |
| SwissProt (Mouse) | P68134 |
| SwissProt (Rat) | P68136 |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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