Art No.: YLD8440
Application: WB, IHC
Size: 50ul/100ul
Reaction: H, M, R
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| Background | Mouse monoclonal antibody to ERK1/2 |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of human ERK1/2. The exact sequence is proprietary. |
| WB description | Western blot analysis of ERK1/2 expression in mouse brain (A), rat brain (B) whole cell lysates. |
| IHC description | Immunohistochemical analysis of ERK1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | |
| Clonality | Monoclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/1000 - 1/2000), IH (1/100 - 1/200) |
| Gene ID(Human) | 5594; 5595 |
| Gene ID (Mouse) | |
| Gene ID(Rat) | |
| SwissProt (Human) | P27361; P28482 |
| SwissProt (Mouse) | |
| SwissProt (Rat) | |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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