Art No.: YLD8444
Application: WB, IF/IC, IP
Size: 50ul/100ul
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| Background | Mouse monoclonal antibody to FLAG-tag |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of FLAG-tag. The exact sequence is proprietary. |
| WB description | Western blot analysis of over-expressed HA-tagged protein in 293T cell lysate. |
| IHC description | |
| IF/ICC description | Immunofluorescent analysis of HA-tag staining in 293T cells transfected with a HA-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
| IP description | Immunoprecipitation of HA-tagged protein from HEK293T cells transfected with vector overexpressing HA tag, using anti-HA-tag Antibody. |
| FC description | |
| ChIP description | |
| Purification_Method | |
| Clonality | Monoclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200) |
| Gene ID(Human) | |
| Gene ID (Mouse) | |
| Gene ID(Rat) | |
| SwissProt (Human) | |
| SwissProt (Mouse) | |
| SwissProt (Rat) | |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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