Art No.: YLD8448
Application: WB, IHC
Size: 50ul/100ul
Reaction: H, M, R
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| Background | Mouse monoclonal antibody to Gamma-enolase |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of human Gamma-enolase. The exact sequence is proprietary. |
| WB description | Western blot analysis of Gamma-enolase expression in Hela (A), Jurkat (B), 293T (C) whole cell lysates. |
| IHC description | Immunohistochemical analysis of Gamma-enolase staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | |
| Clonality | Monoclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/1000 - 1/2000), IH (1/100 - 1/200) |
| Gene ID(Human) | 2026 |
| Gene ID (Mouse) | 13807 |
| Gene ID(Rat) | 100911625 |
| SwissProt (Human) | P09104 |
| SwissProt (Mouse) | P17183 |
| SwissProt (Rat) | P07323 |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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