Art No.: YLD8483
Application: WB, IHC
Size: 50ul/100ul
Reaction: H, M, R
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| Background | Mouse monoclonal antibody to Histone H2B |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of human Histone H2B. The exact sequence is proprietary. |
| WB description | Western blot analysis of Histone H2B expression in Hela (A), NIH3T3 (B), Raw264.7 (C), rat brain (D), rat kidney (E) whole cell lysates. |
| IHC description | Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | |
| Clonality | Monoclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/1000 - 1/3000), IH (1/200 - 1/500) |
| Gene ID(Human) | 3018 |
| Gene ID (Mouse) | 319178 |
| Gene ID(Rat) | |
| SwissProt (Human) | P33778 |
| SwissProt (Mouse) | Q64475 |
| SwissProt (Rat) | |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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