Art No.: YLD8499
Application: WB, IF/IC, IP
Size: 50ul/100ul
Reaction: H, M, R, Yeast
Inquire Now
| Background | Mouse monoclonal antibody to Histone H3 |
| Concentration | 1mg/mL |
| Immunogen | Recombinant protein corresponding to human Histone H3. |
| WB description | Western blot analysis of Histone H3 expression in Hela (A), Raw264.7 (B), mouse brain (C), rat brain (D) whole cell lysates. |
| IHC description | |
| IF/ICC description | Immunofluorescent analysis of Histone H3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
| IP description | Immunoprecipitation of Histone H3 from 0.5mg Hela whole cell extract lysate, using anti-Histone H3 Antibody. |
| FC description | |
| ChIP description | |
| Purification_Method | |
| Clonality | Monoclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/2000 - 1/5000), IF/IC (1/100 - 1/500), IP (1/50 - 1/200) |
| Gene ID(Human) | 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968 |
| Gene ID (Mouse) | 319152; 15077; 15078 |
| Gene ID(Rat) | 291159; 100361558 |
| SwissProt (Human) | P68431; Q71DI3; P84243 |
| SwissProt (Mouse) | P68433; P84228; P84244 |
| SwissProt (Rat) | Q6LED0; P84245 |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
Copyright © Shanghai YL Biotech Co., Ltd All Rights Reserved
Technical Support:
