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Product Name: Mouse anti-Myc-tag Antibody

Art No.: YLD8522

Application: WB, IF/IC, IP

Size: 50ul/100ul

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BackgroundMouse monoclonal antibody to Myc-tag
Concentration1mg/mL
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence of Myc-tag. The exact sequence is proprietary.
WB descriptionWestern blot analysis of over-expressed V5-tagged protein in 293T cell lysate.
IHC description
IF/ICC descriptionImmunofluorescent analysis of V5-tag staining in 293T cells transfected with a V5-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IP descriptionImmunoprecipitation of V5-tagged protein from HEK293T cells transfected with vector overexpressing V5 tag, using  anti-V5-tag Antibody.
FC description
ChIP description
Purification_Method
ClonalityMonoclonal
Conjugation
BufferLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
DilutionWB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200)
Gene ID(Human)
Gene ID (Mouse)
Gene ID(Rat)
SwissProt (Human)
SwissProt (Mouse)
SwissProt (Rat)
StorageStore at -20℃. Avoid repeated freeze / thaw cycles.


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  • Tel.: +86 021 5772 3955
  • Fax: +86 021 5772 3955
  • Orders for consulting: export@ylbiont.com
  • Technical consulting: william@ylbiont.com
  • Add.: 3577 Jinke Road, Zhangjiang Town, Pudong New Area, Shanghai
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