Art No.: YLD8525
Application: WB, IHC, IP
Size: 50ul/100ul
Reaction: H, M, R
Inquire Now
| Background | Mouse monoclonal antibody to NF-kappaB p65 |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of human NF-kappaB p65. The exact sequence is proprietary. |
| WB description | Western blot analysis of NF-kappaB p65 expression in Hela (A), 293T (B), HepG (C) whole cell lysates. |
| IHC description | Immunohistochemical analysis of NF-kappaB p65 staining in mouse hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | Immunoprecipitation of NF-kappaB p65 from 0.5mg Hela whole cell extract lysate, using anti-NF-kappaB p65 Antibody. |
| FC description | |
| ChIP description | |
| Purification_Method | |
| Clonality | Monoclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/1000 - 1/3000), IH (1/100 - 1/200), IP (1/50 - 1/200) |
| Gene ID(Human) | 5970 |
| Gene ID (Mouse) | 19697 |
| Gene ID(Rat) | |
| SwissProt (Human) | Q04206 |
| SwissProt (Mouse) | Q04207 |
| SwissProt (Rat) | |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
Copyright © Shanghai YL Biotech Co., Ltd All Rights Reserved
Technical Support:
