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Product Name: Rabbit anti-CRKL Antibody
Crk-like protein

Art No.: YLD2193

Application: WB, IHC, IF/IC

Size: 50ul/100ul

Reaction: H, M, R

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BackgroundRabbit polyclonal antibody to CRKL
Concentration1mg/mL
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human CRKL. The exact sequence is proprietary.
WB descriptionWestern blot analysis of CRKL expression in HEK293T (A), SGC7901 (B) whole cell lysates.
IHC descriptionImmunohistochemical analysis of CRKL staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC descriptionImmunofluorescent analysis of CRKL staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
IP description
FC description
ChIP description
Purification_MethodThe antibody was purified by immunogen affinity chromatography.
ClonalityPolyclonal
Conjugation
BufferLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
DilutionWB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)
Gene ID(Human)1399
Gene ID (Mouse)12929
Gene ID(Rat)100911248; 287942
SwissProt (Human)P46109
SwissProt (Mouse)P47941
SwissProt (Rat)Q5U2U2
StorageStore at -20℃. Avoid repeated freeze / thaw cycles.


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  • Tel.: +86 021 5772 3955
  • Fax: +86 021 5772 3955
  • Orders for consulting: export@ylbiont.com
  • Technical consulting: william@ylbiont.com
  • Add.: 3577 Jinke Road, Zhangjiang Town, Pudong New Area, Shanghai
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