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Product Name: Rabbit anti-Cyclin A1 Antibody
Cyclin-A1

Art No.: YLD2261

Application: WB, IHC, IF/IC

Size: 50ul/100ul

Reaction: H, M, R, B, C, D, P, Rb

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BackgroundRabbit polyclonal antibody to Cyclin A1
Concentration1mg/mL
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cyclin A1. The exact sequence is proprietary.
WB descriptionWestern blot analysis of Cyclin A1 expression in MCF7 (A), NIH3T3 (B) whole cell lysates.
IHC descriptionImmunohistochemical analysis of Cyclin A1 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC descriptionImmunofluorescent analysis of Cyclin A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
IP description
FC description
ChIP description
Purification_MethodThe antibody was purified by immunogen affinity chromatography.
ClonalityPolyclonal
Conjugation
BufferLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
DilutionWB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene ID(Human)8900
Gene ID (Mouse)12427
Gene ID(Rat)295052
SwissProt (Human)P78396
SwissProt (Mouse)Q61456
SwissProt (Rat)Q6AY13
StorageStore at -20℃. Avoid repeated freeze / thaw cycles.


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  • Tel.: +86 021 5772 3955
  • Fax: +86 021 5772 3955
  • Orders for consulting: export@ylbiont.com
  • Technical consulting: william@ylbiont.com
  • Add.: 3577 Jinke Road, Zhangjiang Town, Pudong New Area, Shanghai
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