Art No.: YLD0652
Application: WB, IHC
Size: 50ul/100ul
Reaction: H, M, R
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| Background | Rabbit polyclonal antibody to ATF4 |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATF4. The exact sequence is proprietary. |
| WB description | Western blot analysis of ATF4 expression in MCF7 (A), mouse brain (B), rat brain (C) whole cell lysates. |
| IHC description | Immunohistochemical analysis of ATF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | The antibody was purified by immunogen affinity chromatography. |
| Clonality | Polyclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/500 - 1/1000), IH (1/100 - 1/200) |
| Gene ID(Human) | 468 |
| Gene ID (Mouse) | 11911 |
| Gene ID(Rat) | 79255 |
| SwissProt (Human) | P18848 |
| SwissProt (Mouse) | Q06507 |
| SwissProt (Rat) | Q9ES19 |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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