Art No.: YLD0671
Application: WB, IHC
Size: 50ul/100ul
Reaction: H, M, R
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| Background | Rabbit polyclonal antibody to ATG7 |
| Concentration | 1mg/mL |
| Immunogen | Recombinant full length protein of human ATG7 |
| WB description | Western blot analysis of ATG7 expression in THP1 (A), HeLa (B), mouse kidney (C), rat liver (D) whole cell lysates. |
| IHC description | Immunohistochemical analysis of ATG7 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | The antibody was purified by immunogen affinity chromatography. |
| Clonality | Polyclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/500 - 1/2000), IH (1/50 - 1/200) |
| Gene ID(Human) | 10533 |
| Gene ID (Mouse) | 74244 |
| Gene ID(Rat) | 312647 |
| SwissProt (Human) | O95352 |
| SwissProt (Mouse) | Q9D906 |
| SwissProt (Rat) | Q641Y5 |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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