Art No.: YLD0693
Application: WB, IHC
Reaction: H, M, R
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| Background | Rabbit polyclonal antibody to ATP5B |
| Concentration | 1mg/mL |
| Immunogen | Recombinant full length protein of human ATP5B |
| WB description | Western blot analysis of ATP5B expression in SW620 (A), HepG2 (B), mouse kidney (C), mouse brain (D) whole cell lysates. |
| IHC description | Immunohistochemical analysis of ATP5B staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | The antibody was purified by immunogen affinity chromatography. |
| Clonality | Polyclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/500 - 1/2000), IH (1/50 - 1/200) |
| Gene ID(Human) | 506 |
| Gene ID (Mouse) | 11947 |
| Gene ID(Rat) | 171374 |
| SwissProt (Human) | P06576 |
| SwissProt (Mouse) | P56480 |
| SwissProt (Rat) | P10719 |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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