Art No.: YLD2352
Application: WB, IF/IC
Size: 50ul/100ul
Reaction: H, M, R, D, Mk, Rb
Inquire NowBackground | Rabbit polyclonal antibody to Cytochrome P450 2E1 |
Concentration | 1mg/mL |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytochrome P450 2E1. The exact sequence is proprietary. |
WB description | Western blot analysis of Cytochrome P450 2E1 expression in HEK293T (A), NIH3T3 (B), H9C2 (C), Human Liver (D) whole cell lysates. |
IHC description | |
IF/ICC description | Immunofluorescent analysis of Cytochrome P450 2E1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
IP description | |
FC description | |
ChIP description | |
Purification_Method | The antibody was purified by immunogen affinity chromatography. |
Clonality | Polyclonal |
Conjugation | |
Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Dilution | WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
Gene ID(Human) | 1571 |
Gene ID (Mouse) | 13106 |
Gene ID(Rat) | 25086 |
SwissProt (Human) | P05181 |
SwissProt (Mouse) | Q05421 |
SwissProt (Rat) | P05182 |
Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |