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Product Name: Rabbit anti-BCKDK Antibody
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN

Art No.: YLD0803

Application: WB, IHC, IF/IC

Size: 50ul/100ul

Reaction: H, M, R, B, D, Mk, P

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BackgroundRabbit polyclonal antibody to BCKDK
Concentration1mg/mL
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human BCKDK. The exact sequence is proprietary.
WB descriptionWestern blot analysis of BCKDK expression in Raji (A), HeLa (B), Jurkat (C), SHSY5Y (D) whole cell lysates.
IHC descriptionImmunohistochemical analysis of BCKDK staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC descriptionImmunofluorescent analysis of BCKDK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IP description
FC description
ChIP description
Purification_MethodThe antibody was purified by immunogen affinity chromatography.
ClonalityPolyclonal
Conjugation
BufferLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
DilutionWB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene ID(Human)10295
Gene ID (Mouse)12041
Gene ID(Rat)29603
SwissProt (Human)O14874
SwissProt (Mouse)O55028
SwissProt (Rat)Q00972
StorageStore at -20℃. Avoid repeated freeze / thaw cycles.


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  • Tel.: +86 021 5772 3955
  • Fax: +86 021 5772 3955
  • Orders for consulting: export@ylbiont.com
  • Technical consulting: william@ylbiont.com
  • Add.: 3577 Jinke Road, Zhangjiang Town, Pudong New Area, Shanghai
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