Home>> Products >> Antibody >> Polyclonal Antibodies >> Rabbit anti-BCL2 Antibody
  • Tel.: +86 021 5772 3955
  • Fax: +86 021 5772 3955
  • Orders for consulting: export@ylbiont.com
  • Technical consulting: william@ylbiont.com
  • Add.: 3577 Jinke Road, Zhangjiang Town, Pudong New Area, Shanghai

Product Name: Rabbit anti-BCL2 Antibody
Apoptosis regulator Bcl-2

Art No.: YLD0811

Application: WB, IHC, IF/IC

Size: 50ul/100ul

Reaction: H

Inquire Now
BackgroundRabbit polyclonal antibody to BCL2
Concentration1mg/mL
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL2. The exact sequence is proprietary.
WB descriptionWestern blot analysis of BCL2 expression in HeLa (A), A2780 (B) whole cell lysates.
IHC descriptionImmunohistochemical analysis of BCL2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC descriptionImmunofluorescent analysis of BCL2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IP description
FC description
ChIP description
Purification_MethodThe antibody was purified by immunogen affinity chromatography.
ClonalityPolyclonal
Conjugation
BufferLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
DilutionWB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Gene ID(Human)596
Gene ID (Mouse)
Gene ID(Rat)
SwissProt (Human)P10415
SwissProt (Mouse)
SwissProt (Rat)
StorageStore at -20℃. Avoid repeated freeze / thaw cycles.


Copyright © Shanghai YL Biotech Co., Ltd All Rights Reserved

Technical Support:

Contact Us
  • Tel.: +86 021 5772 3955
  • Fax: +86 021 5772 3955
  • Orders for consulting: export@ylbiont.com
  • Technical consulting: william@ylbiont.com
  • Add.: 3577 Jinke Road, Zhangjiang Town, Pudong New Area, Shanghai
Top