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Product Name: Rabbit anti-delta-Sarcoglycan Antibody
Delta-sarcoglycan; Delta-SG; 35 kDa dystrophin-associated glycoprotein; 35DAG

Art No.: YLD2474

Application: WB, IHC, IF/IC

Size: 50ul/100ul

Reaction: H, M, R

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BackgroundRabbit polyclonal antibody to delta-Sarcoglycan
Concentration1mg/mL
ImmunogenRecombinant full length protein of human delta-Sarcoglycan
WB descriptionWestern blot analysis of delta-Sarcoglycan expression in Jurkat (A), THP1 (B), mouse skeletal muscle (C), rat heart (D) whole cell lysates.
IHC descriptionImmunohistochemical analysis of delta-Sarcoglycan staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC descriptionImmunofluorescent analysis of delta-Sarcoglycan staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
IP description
FC description
ChIP description
Purification_MethodThe antibody was purified by immunogen affinity chromatography.
ClonalityPolyclonal
Conjugation
BufferLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
DilutionWB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)
Gene ID(Human)6444
Gene ID (Mouse)24052
Gene ID(Rat)
SwissProt (Human)Q92629
SwissProt (Mouse)P82347
SwissProt (Rat)
StorageStore at -20℃. Avoid repeated freeze / thaw cycles.


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  • Tel.: +86 021 5772 3955
  • Fax: +86 021 5772 3955
  • Orders for consulting: export@ylbiont.com
  • Technical consulting: william@ylbiont.com
  • Add.: 3577 Jinke Road, Zhangjiang Town, Pudong New Area, Shanghai
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