Art No.: YLD0838
Application: WB, IHC, IF/IC, IP
Size: 50ul/100ul
Reaction: H, M, R
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| Background | Rabbit polyclonal antibody to Beclin-1 |
| Concentration | 1mg/mL |
| Immunogen | Recombinant full length protein of human Beclin-1 |
| WB description | Western blot analysis of Beclin-1 expression in A431 (A), MCF7 (B), Raji (C), mouse kidney (D), rat brain (E) whole cell lysates. |
| IHC description | Immunohistochemical analysis of Beclin-1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | The antibody was purified by immunogen affinity chromatography. |
| Clonality | Polyclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/20 - 1/50) |
| Gene ID(Human) | 8678 |
| Gene ID (Mouse) | 56208 |
| Gene ID(Rat) | 114558 |
| SwissProt (Human) | Q14457 |
| SwissProt (Mouse) | O88597 |
| SwissProt (Rat) | Q91XJ1 |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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