Art No.: YLD0954
Application: WB, IHC, IP
Size: 50ul/100ul
Reaction: H, M, R, C
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| Background | Rabbit polyclonal antibody to B-RAF (pS446) |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human B-RAF. The exact sequence is proprietary. |
| WB description | Western blot analysis of B-RAF (pS446) expression in K562 (A), HEK293T (B) whole cell lysates. |
| IHC description | Immunohistochemical analysis of B-RAF (pS446) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | The antibody was purified by immunogen affinity chromatography. |
| Clonality | Polyclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100) |
| Gene ID(Human) | 673 |
| Gene ID (Mouse) | 109880 |
| Gene ID(Rat) | |
| SwissProt (Human) | P15056 |
| SwissProt (Mouse) | P28028 |
| SwissProt (Rat) | |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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