Art No.: YLD5502
Application: WB, IHC, IF/IC, ChIP
Size: 50ul/100ul
Reaction: H, M, R, B, P
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| Background | Rabbit polyclonal antibody to NF-kappaB p65 (AcK310) |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p65 (AcK310). The exact sequence is proprietary. |
| WB description | Western blot analysis of S6K1 (pS434) expression in K562 insulin-treated (A), mouse brain (B), rat brain (C) whole cell lysates. |
| IHC description | Immunohistochemical analysis of S6K1 (pS434) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
| IF/ICC description | Immunofluorescent analysis of S6K1 (pS434) staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | The antibody was purified by immunogen affinity chromatography. |
| Clonality | Polyclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) |
| Gene ID(Human) | 6198 |
| Gene ID (Mouse) | 72508 |
| Gene ID(Rat) | 83840 |
| SwissProt (Human) | P23443 |
| SwissProt (Mouse) | Q8BSK8 |
| SwissProt (Rat) | P67999 |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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