Art No.: YLD5989
Application: WB, IF/IC
Size: 50ul/100ul
Reaction: H, M, R, B, C, D, Mk, P, Rb
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| Background | Rabbit polyclonal antibody to PCNA |
| Concentration | 1mg/mL |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary. |
| WB description | Western blot analysis of PCNA expression in HeLa (A), SP2/0 (B), H9C2 (C) whole cell lysates. |
| IHC description | |
| IF/ICC description | Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
| IP description | |
| FC description | |
| ChIP description | |
| Purification_Method | The antibody was purified by immunogen affinity chromatography. |
| Clonality | Polyclonal |
| Conjugation | |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Dilution | WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) |
| Gene ID(Human) | 5111 |
| Gene ID (Mouse) | 18538 |
| Gene ID(Rat) | 25737 |
| SwissProt (Human) | P12004 |
| SwissProt (Mouse) | P17918 |
| SwissProt (Rat) | P04961 |
| Storage | Store at -20℃. Avoid repeated freeze / thaw cycles. |
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